DCFDA - Cellular Reactive Oxygen Species Detection Assay Kit

ROS assay cell type - H9c2 rat cardiomyocytes

Experiment
ROS assay cell type - H9c2 rat cardiomyocytes
Product
DCFDA - Cellular Reactive Oxygen Species Detection Assay Kit from Abcam
Manufacturer
Abcam

Protocol tips

Protocol tips
- Cells were treated with DCFDA.

- After diffusion into the cells, DCFDA was deacetylated by cellular esterases to a non-fluorescent compound, which was later oxidized by ROS into 2′,7′-dichlorofluorescin (DCF)

Publication protocol

Cellular ROS was measured using a detection assay kit (Abcam, Cambridge, MA, USA). In brief, 2′,7′-dichlorofluorescein diacetate (DCFDA), a fluorogenic dye that measures hydroxyl, peroxyl and other ROS activity within the cell, was added to the cells growing in the 96-well plates. After diffusion into the cells, DCFDA was deacetylated by cellular esterases to a non-fluorescent compound, which was later oxidized by ROS into 2′,7′-dichlorofluorescin (DCF), a highly fluorescent compound. Fluorescence from the DCF was detected by fluorescence micro plate reader with maximum excitation and emission spectra of 495 nm and 529 nm, respectively.

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Papers

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Paper title
Metformin Protects Cardiomyocyte from Doxorubicin Induced Cytotoxicity through an AMP-Activated Protein Kinase Dependent Signaling Pathway: An Study
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Manufacturer protocol

Download the product protocol from Abcam for DCFDA - Cellular Reactive Oxygen Species Detection Assay Kit below.

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