DCFDA - Cellular Reactive Oxygen Species Detection Assay Kit

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	- Cells were treated with DCFDA. - After diffusion into the cells, DCFDA was deacetylated by cellular esterases to a non-fluorescent compound, which was later oxidized by ROS into 2′,7′-dichlorofluorescin (DCF)

Protocol tips
- Cells were treated with DCFDA. - After diffusion into the cells, DCFDA was deacetylated by cellular esterases to a non-fluorescent compound, which was later oxidized by ROS into 2′,7′-dichlorofluorescin (DCF)

Publication protocol

Cellular ROS was measured using a detection assay kit (Abcam, Cambridge, MA, USA). In brief, 2′,7′-dichlorofluorescein diacetate (DCFDA), a fluorogenic dye that measures hydroxyl, peroxyl and other ROS activity within the cell, was added to the cells growing in the 96-well plates. After diffusion into the cells, DCFDA was deacetylated by cellular esterases to a non-fluorescent compound, which was later oxidized by ROS into 2′,7′-dichlorofluorescin (DCF), a highly fluorescent compound. Fluorescence from the DCF was detected by fluorescence micro plate reader with maximum excitation and emission spectra of 495 nm and 529 nm, respectively.

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