LIVE/DEAD™ Viability/Cytotoxicity Kit, for mammalian cells

Protocol tips

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	Add Calcein AM (0.5 μM) and ethidium homodimer-1 (1 μM) Incubate the cells for 30–45 minutes at room temperature

Protocol tips
Add Calcein AM (0.5 μM) and ethidium homodimer-1 (1 μM) Incubate the cells for 30–45 minutes at room temperature

Publication protocol

The viability of cells in 3D and 2D cultures was determined on days 1, 4 and 7 using a live/dead® viability/cytotoxicity kit according to the manufacturer’s recommendations. Calcein AM (0.5 μM) and ethidium homodimer-1 (1 μM) were used to stain viable and dying cells, respectively. The hydrogel/cell constructs were observed using an inverted fluorescence microscope (Leica, Wetzlar, Germany). The experiments were repeated three times.

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Manufacturer protocol

Download the product protocol from Thermo Fisher Scientific for LIVE/DEAD™ Viability/Cytotoxicity Kit, for mammalian cells below.

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