PE Mouse Anti-Human CD140a

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		Cytometry and sorting was performed using a BD FACSAria (Becton Dickinson).

Downstream tips
Cytometry and sorting was performed using a BD FACSAria (Becton Dickinson).

Publication protocol

Cytometry and sorting was performed using a BD FACSAria (Becton Dickinson). For CD133/A2B5, cells were stained with CD133-APC (Miltentyi Biotech) and A2B5 IgM (Clone 105; ATCC), and goat anti-mouse IgM F(ab′) PerCP (Jackson ImmunoResearch) or goat anti-mouse IgM A488 (Invitrogen). For CD133/CD140a, cells were stained with CD140a-PE (BD Pharmingen) and CD133-APC. Matched fluorescence minus-one controls were used to set gates following doublet discrimination. The incidence of CD133- and CD140a-positive cells was compared by one-way analysis of variance (ANOVA), followed by the Tukey's multiple comparison test (*** indicates P<0.001 vs. CD133+CD140a− cells). Typically from 108 stained cells, we obtained between 0.5–1×106 CD133+CD140a− cells and <3×105 CD133+CD140a+ cells.

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