PDGFR-α Antibody (C-9): sc-398206

Protocol tips

Upstream tips	Protocol tips	Downstream tips
	For flow cytometry analysis, cultured cells were trypsinized and resuspended in in PBS with 2% FBS and 5 mM EDTA, and stained with primary antibody for 30 min at 4 °C.	Stained cells were analyzed by FACSCantoTM II (BD Biosciences).

Protocol tips
For flow cytometry analysis, cultured cells were trypsinized and resuspended in in PBS with 2% FBS and 5 mM EDTA, and stained with primary antibody for 30 min at 4 °C.
Downstream tips
Stained cells were analyzed by FACSCantoTM II (BD Biosciences).

Publication protocol

For flow cytometry analysis, cultured cells were trypsinized and resuspended in in PBS with 2% FBS and 5 mM EDTA, and stained with primary antibody for 30 min at 4 °C. Primary antibodies used were anti PDGFRα (1:200; SC-338, Santa Cruz). Secondary antibodies used Alexa Fluor 647 conjugated IgG (1:400; 111-606-003, Jackson immunoResearch) for 30 min at 4 °C in the dark. Stained cells were analyzed by FACSCantoTM II (BD Biosciences).

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