PE Mouse Anti-Human CD31

Protocol tips

Upstream tips	Protocol tips	Downstream tips
	MV in 50 μl aliquots were labeled.	quantified by FACS (FACSCanto™) with a size > 150 nm as described previously

Protocol tips
MV in 50 μl aliquots were labeled.
Downstream tips
quantified by FACS (FACSCanto™) with a size > 150 nm as described previously

Publication protocol

MV in 50 μl aliquots were labeled with annexin V-FITC, paired with a PE-conjugated antibody against either PE CAM-1, integrin avβ3, ICAM-1, E-selectin, or MCAM, then quantified by FACS (FACSCanto™) with a size > 150 nm as described previously [33, 36]. The total numbers of each MV antigenic phenotype per flask of conditioned medium were determined. The fold increase in number above control (unstimulated) conditions was determined for each adhesion molecule and stimulus.

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Manufacturer protocol

Download the product protocol from BD Biosciences for PE Mouse Anti-Human CD31 below.

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