CD38 Monoclonal Antibody (HIT2)

Protocol tips

Upstream tips	Protocol tips	Downstream tips
Fluorescently labelled antibodies were validated for their sensitivity using monocyte derived macrophages

Upstream tips
Fluorescently labelled antibodies were validated for their sensitivity using monocyte derived macrophages

Publication protocol

Fluorescently labelled antibodies were validated for their sensitivity using monocyte derived macrophages (explanation in online supplement and shown in Table S6). Antibodies chosen for macrophage subpopulation phenotyping were: CD14 (61D3), CD38 (HIT2), HLA-DR (LN3), CD163 (GH1/61), CD206 (19.2) (all eBioscience, Hatfield, UK), and CD36 (CB38) (BD Biosciences, Oxford, United Kingdom). Flow cytometry method details are in the online supplement. Data are expressed as the percentage of positive cells or median fluorescence intensity (MFI).

Full paper   Login or join for free to view the full paper.

Reviews

Discussion

Papers

Check out relevant papers found by Labettor's AI that are relevant for performing Flow cytometry Anti-bodies Human - CD38 using CD38 Monoclonal Antibody (HIT2) from Thermo Fisher Scientific.

Manufacturer protocol

Download the product protocol from Thermo Fisher Scientific for CD38 Monoclonal Antibody (HIT2) below.

Download manufacturer protocol

Videos

Check out videos that might be relevant for performing Flow cytometry Anti-bodies Human - CD38 using CD38 Monoclonal Antibody (HIT2) from Thermo Fisher Scientific. Please note that these videos are representative and steps or experiment specific processes must be kept in mind to expect desired results.