HLA-DR Monoclonal Antibody (LN3), eBioscience™

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	Fluorescently labelled antibodies were validated for their sensitivity using monocyte derived macrophages

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Fluorescently labelled antibodies were validated for their sensitivity using monocyte derived macrophages

Publication protocol

Fluorescently labelled antibodies were validated for their sensitivity using monocyte derived macrophages (explanation in online supplement and shown in Table S6). Antibodies chosen for macrophage subpopulation phenotyping were: CD14 (61D3), CD38 (HIT2), HLA-DR (LN3), CD163 (GH1/61), CD206 (19.2) (all eBioscience, Hatfield, UK), and CD36 (CB38) (BD Biosciences, Oxford, United Kingdom). Flow cytometry method details are in the online supplement. Data are expressed as the percentage of positive cells or median fluorescence intensity (MFI).

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