CD70 Monoclonal Antibody (BU69), FITC

Protocol tips

Upstream tips	Protocol tips	Downstream tips
	cells (2×106) along with blank controls were firstly centrifuged at 2 000 rpm for 10 min, rinsed in PBS, and Ab added. After mixture and washing, cells were fixed by 0.5 mL paraformaldehyde and loaded on flow cytometry for detection.

Protocol tips
cells (2×106) along with blank controls were firstly centrifuged at 2 000 rpm for 10 min, rinsed in PBS, and Ab added. After mixture and washing, cells were fixed by 0.5 mL paraformaldehyde and loaded on flow cytometry for detection.

Publication protocol

Flow cytometry was used to detect the protein expression level of CD70 and CD11a in CD4+ T cells. In brief, 45 hours after transfection, cells (2×106) along with blank controls were firstly centrifuged at 2 000 rpm for 10 min, rinsed in PBS, and added with FITC-CD11a or FITC-CD70 conjugated antibodies (eBioscience, US). After mixture and washing, cells were fixed by 0.5 mL paraformaldehyde and loaded on flow cytometry for detection.

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Manufacturer protocol

Download the product protocol from eBioscience for CD70 Monoclonal Antibody (BU69), FITC below.

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