PE Mouse Anti-Human CD26 Clone L272

Protocol tips

Upstream tips	Protocol tips	Downstream tips
	Samples were processed using a standard lyse/wash surface staining procedure with FACS Lyse solution according to the manufacturer's protocol (BDIS,www.bdbiosciences.com). Caltag Fix and Perm reagent kit (Invitrogen, Caltag, Catalogue No. GAS‐004) was used for intracellular staining. The 1 × 106 leukocytes in 100 μL of sample were stained with an appropriate volume of antibodies in each tube.	All samples were analyzed using the Euroflow 8‐color immunophenotyping panel (Table 1) on the FACSCantoII cytometer (Becton Dickinson, San Jose, California). Instrument set‐up and compensation matrix was established using CS&T and FACS Comp beads as per the manufacturer's recommendations.

Protocol tips
Samples were processed using a standard lyse/wash surface staining procedure with FACS Lyse solution according to the manufacturer's protocol (BDIS,www.bdbiosciences.com). Caltag Fix and Perm reagent kit (Invitrogen, Caltag, Catalogue No. GAS‐004) was used for intracellular staining. The 1 × 106 leukocytes in 100 μL of sample were stained with an appropriate volume of antibodies in each tube.
Downstream tips
All samples were analyzed using the Euroflow 8‐color immunophenotyping panel (Table 1) on the FACSCantoII cytometer (Becton Dickinson, San Jose, California). Instrument set‐up and compensation matrix was established using CS&T and FACS Comp beads as per the manufacturer's recommendations.

Publication protocol

FC immunophenotyping studies were performed on BM or PB samples from patients with suspected NK‐cell malignancies. All samples were analyzed using the Euroflow 8‐color immunophenotyping panel (Table 1) on the FACSCantoII cytometer (Becton Dickinson, San Jose, California). Instrument set‐up and compensation matrix was established using CS&T and FACS Comp beads as per the manufacturer's recommendations. Samples were processed using a standard lyse/wash surface staining procedure with FACS Lyse solution according to the manufacturer's protocol (BDIS,www.bdbiosciences.com). Caltag Fix and Perm reagent kit (Invitrogen, Caltag, Catalogue No. GAS‐004) was used for intracellular staining. The 1 × 106 leukocytes in 100 μL of sample were stained with an appropriate volume of antibodies in each tube.

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Manufacturer protocol

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