CD33 Monoclonal Antibody (WM-53 (WM53)), PE, eBioscience™

Protocol tips

Upstream tips	Protocol tips	Downstream tips
All samples were processed within 12 h from collection. Whole blood was lysed prior to staining using the RBC Lysis solution (Qiagen, Germany) according to manufacturer's specification.

Upstream tips
All samples were processed within 12 h from collection. Whole blood was lysed prior to staining using the RBC Lysis solution (Qiagen, Germany) according to manufacturer's specification.

Publication protocol

All samples were processed within 12 h from collection. Whole blood was lysed prior to staining using the RBC Lysis solution (Qiagen, Germany) according to manufacturer's specification. Immune populations were identified by staining with fluorophore-conjugated anti-CD11b, anti-CD33, anti-CD3, anti-HLA-DR, anti-CD45, anti-CD68, anti-CD206, anti-CD163, (BioLegend), anti-CD14, anti-CD15 (eBioscience) antibodies on ice for 30 min. Fluorescence data was acquired using BD Accuri C6 (BD Biosciences), Cyan and/or CytoFLEX (Beckman Coulter) cytometers. Normalised population statistics –including the median fluorescence intensities (MFI) were determined using FlowJo (BD Biosciences, formerly developed by FlowJo LLC).

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Manufacturer protocol

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