FITC Mouse Anti-Human CD51/CD61

Protocol tips

Upstream tips	Protocol tips	Downstream tips
	Measurements of EPCs and MPs were performed as previously reported, using fresh blood samples in EDTA containing tubes.	For determination of EPCs, a minimum of 500,000 events was acquired by flow-cytometry (FACSCalibur, BD Biosciences, USA)

Protocol tips
Measurements of EPCs and MPs were performed as previously reported, using fresh blood samples in EDTA containing tubes.
Downstream tips
For determination of EPCs, a minimum of 500,000 events was acquired by flow-cytometry (FACSCalibur, BD Biosciences, USA)

Publication protocol

Measurements of EPCs and MPs were performed as previously reported, using fresh blood samples in EDTA containing tubes.12-15 For determination of EPCs, a minimum of 500,000 events was acquired by flow-cytometry (FACSCalibur, BD Biosciences, USA). Fluorescently labeled mouse anti-human antibodies were used for EPCs (CD34 FITC, BD Biosciences, USA; CD133 APC, Miltenyi Biotec, USA; KDR PE, R&D Systems, USA), PMPs (CD42 FITC and CD31 PE, BD Biosciences, USA) and EMPs (CD51 FITC, BD Biosciences). Disposable containers (BD Biosciences) were used to quantify the number of microparticles per microliter of platelet-poor plasma (PPP).

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