PE Mouse anti-Human CD96

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A total of 2 to 5 mL bone marrow was extracted with EDTA anticoagulation from AML patients before chemotherapy. The marrow was treated by density gradient centrifugation in Ficoll-Paque PREMIUM to isolate mononuclear cells.

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A total of 2 to 5 mL bone marrow was extracted with EDTA anticoagulation from AML patients before chemotherapy. The marrow was treated by density gradient centrifugation in Ficoll-Paque PREMIUM to isolate mononuclear cells.

Publication protocol

A total of 2 to 5 mL bone marrow was extracted with EDTA anticoagulation from AML patients before chemotherapy. The marrow was treated by density gradient centrifugation in Ficoll-Paque PREMIUM to isolate mononuclear cells. Four colors (FITC, PE, PerCP, and APC) fluorescent antibodies were used to label the cells. FSC/SSC was set to exclude debris, SSC/CD45 was set to select white blood cells, and CD34+CD38− was set to select hematopoietic stem cells. Furthermore, CD123+ was set among CD34+CD38- hematopoietic stem cells to identify LSC. CD33, CD96, and DAPK expression was analyzed in CD34+CD38−CD123+ LSC. Positive cell rate ≥20% was considered as positive expression in LSC.

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