Autophagy Detection Kit

Protocol tips

Upstream tips	Protocol tips	Downstream tips
	Incubate cells with CYTO-ID dye (2μl of CYTO-ID in 1ml of 1X assay buffer) for 30 min	Incase of Low CYTO-ID Green dye staining in all treatments, increase the reagent concentration (500X dilution of the dye is recommended) and the incubation time. Incase of High CYTO-ID Green staining, it could be that the cell culture medium was depleted of nutrients and so change media 4-8 hours before the experimen

Protocol tips
Incubate cells with CYTO-ID dye (2μl of CYTO-ID in 1ml of 1X assay buffer) for 30 min
Downstream tips
Incase of Low CYTO-ID Green dye staining in all treatments, increase the reagent concentration (500X dilution of the dye is recommended) and the incubation time. Incase of High CYTO-ID Green staining, it could be that the cell culture medium was depleted of nutrients and so change media 4-8 hours before the experimen

Publication protocol

The Cyto-ID® Autophagy Detection Kit (Enzo Life Science, NY, USA) measures autophagic vacuoles and monitors autophagic flux in live cells using a cationic amphiphilic dye that selectively labels autophagic vacuoles. We determined the percentage of Cyto-ID-positive cells according to manufacturer's instructions.

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Manufacturer protocol

Download the product protocol from Abcam for Autophagy Detection Kit below.

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