Human Thrombopoietin R/Tpo R APC-conjugated Antibody

Protocol tips

Upstream tips	Protocol tips	Downstream tips
	Mononuclear cells were analyzed via flow cytometry (CyFlow Space, Partec) immediately after their isolation from blood.	. At least 1 × 106 cells were stained for each antibody, with at least 5 × 105 events captured and analyzed. Unstained and single stain controls were used for compensation and gating. Final gates were based on FSC/SSC and two parameter plots.

Protocol tips
Mononuclear cells were analyzed via flow cytometry (CyFlow Space, Partec) immediately after their isolation from blood.
Downstream tips
. At least 1 × 106 cells were stained for each antibody, with at least 5 × 105 events captured and analyzed. Unstained and single stain controls were used for compensation and gating. Final gates were based on FSC/SSC and two parameter plots.

Publication protocol

Mononuclear cells were analyzed via flow cytometry (CyFlow Space, Partec) immediately after their isolation from blood. The following antibodies were used in the analysis: CD34 (FAB7227G; R&D Systems), CD110 (FAB1016A; R&D Systems), CD38 (FAB2404A; R&D Systems), CD41 (FAB7616A; R&D Systems), CD4 (FAB3791F; R&D Systems), CD8 (FAB1509A; R&D Systems), CD11c (FAB1777N; R&D Systems), CD123 (FAB301P; R&D Systems), and CD3/CD(15+56) cocktail (319101; BioLegend). Antibody concentrations used reflect manufacturer recommendations. At least 1 × 106 cells were stained for each antibody, with at least 5 × 105 events captured and analyzed. Unstained and single stain controls were used for compensation and gating. Final gates were based on FSC/SSC and two parameter plots.

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Manufacturer protocol

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