PE anti-human CD114 (G-CSFR) Antibody

Protocol tips

Upstream tips	Protocol tips	Downstream tips
	Live cell discrimination was determined by the absence of DAPI staining.	Flow cytometry for cell lines was conducted on a FACSCanto II 3-laser flow cytometer (BD Biosciences). FlowJo 10.0 (FlowJo) software was used to analyze flow cytometry data.

Protocol tips
Live cell discrimination was determined by the absence of DAPI staining.
Downstream tips
Flow cytometry for cell lines was conducted on a FACSCanto II 3-laser flow cytometer (BD Biosciences). FlowJo 10.0 (FlowJo) software was used to analyze flow cytometry data.

Publication protocol

Flow cytometry for cell lines was conducted on a FACSCanto II 3-laser flow cytometer (BD Biosciences). FlowJo 10.0 (FlowJo) software was used to analyze flow cytometry data. All samples were gated using an unstained control as reference. PE-conjugated anti-CD114 antibodies (BioLegend, #346106), APC-conjugated anti-CD133 antibodies (BioLegend, #372805), and V450-conjugated anti-CD15 antibodies (BD Biosciences, #561584) were used for flow cytometry and fluorescence-activated cell sorting (FACS) as detailed below. Live cell discrimination was determined by the absence of DAPI staining. Flow cytometry of cells from tumor samples was performed on an LSFortessa X20 flow cytometer (BD Biosciences) as above with live cell discrimination determined by the absence of 7-AAD staining.

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