Cell Proliferation Reagent WST-1

Protocol tips

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- 3,000/well were seeded in a 96 well plate. - Cells were pretreated with siRNA before performing an assay

Upstream tips
- 3,000/well were seeded in a 96 well plate. - Cells were pretreated with siRNA before performing an assay

Publication protocol

HCC cells proliferation was monitored using Cell Proliferation Reagent Kit I (MTT) (Roche Applied Science). siRNA-CCAT1 and empty vector-transfected HepG2 and SNU423 cells (3,000/well) were allowed to grow in 96-well plates. Cell proliferation was measured every 24 h following the manufacturer’s protocol

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Manufacturer protocol

Download the product protocol from Sigma-Aldrich for Cell Proliferation Reagent WST-1 below.

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