CellTiter 96® AQueous One Solution Cell Proliferation Assay (MTS)

Protocol tips

Upstream tips	Protocol tips	Downstream tips
- Completely thaw the 20ml size One Solution Reagent approximately 90 minutes at room temperature, or 10 minutes in a water bath at 37°C.	- Cells were incubated with 20 μl of MTS for 3 h	- To measure the absorbance later, add 25µl of 10% SDS to each well to stop the reaction. Store SDS-treated plates protected from light in a humidified chamber at room temperature for up to 18 hours.

Upstream tips
- Completely thaw the 20ml size One Solution Reagent approximately 90 minutes at room temperature, or 10 minutes in a water bath at 37°C.
Protocol tips
- Cells were incubated with 20 μl of MTS for 3 h
Downstream tips
- To measure the absorbance later, add 25µl of 10% SDS to each well to stop the reaction. Store SDS-treated plates protected from light in a humidified chamber at room temperature for up to 18 hours.

Publication protocol

The cell viability was determined by the 3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium, inner salt (MTS) assay (CellTiter 96® AQueous One Solution Cell Proliferation assay, Promega, Madison, USA) as reported previously [7]. Briefly, cells were cultured in 96-well plates and treated with EGCG (0, 1.56, 3.125, 6.25, 12.5, 25, and 50 μM) for 2 h before being incubated in LPS (250 ng/ml) for another 48 h. Then, the treated cells were incubated with 20 μl of MTS for 3 h. The absorbance was measured at 490 nm with an automatic microplate reader (Tecan, Männedorf, Switzerland). Cell viability was calculated by the following formula: cell viability (fold) = the absorbance of treated group/the average absorbance of untreated group

Full paper   Login or join for free to view the full paper.

Reviews

Discussion

Papers

Check out relevant papers found by Labettor's AI that are relevant for performing Cell cytotoxicity / Proliferation assay cell type - L-02 using CellTiter 96® AQueous One Solution Cell Proliferation Assay (MTS) from Promega.

Manufacturer protocol

Download the product protocol from Promega for CellTiter 96® AQueous One Solution Cell Proliferation Assay (MTS) below.

Download manufacturer protocol

Videos

Check out videos that might be relevant for performing Cell cytotoxicity / Proliferation assay cell type - L-02 using CellTiter 96® AQueous One Solution Cell Proliferation Assay (MTS) from Promega. Please note that these videos are representative and steps or experiment specific processes must be kept in mind to expect desired results.