SmaI R6121

Protocol tips

Upstream tips	Protocol tips	Downstream tips
	HindIII, EcoRI (Boehringer Mannheim), SmaI (Promega). All enzymes at 10 U μl−1. In the lower lanes (Airmail), DNA was digested with restriction enzymes that had been sent by conventional airmail in a plain envelope from Edinburgh, UK, to Boston, MA, and returned via the same route.

Protocol tips
HindIII, EcoRI (Boehringer Mannheim), SmaI (Promega). All enzymes at 10 U μl−1. In the lower lanes (Airmail), DNA was digested with restriction enzymes that had been sent by conventional airmail in a plain envelope from Edinburgh, UK, to Boston, MA, and returned via the same route.

Publication protocol

HindIII, EcoRI (Boehringer Mannheim), SmaI (Promega). All enzymes at 10 U μl−1. In the lower lanes (Airmail), DNA was digested with restriction enzymes that had been sent by conventional airmail in a plain envelope from Edinburgh, UK, to Boston, MA, and returned via the same route. In the upper lanes (−20°C) digestion was performed with samples of the same enzymes stored at −20°C over the time course. The apparent stability of SmaI is particularly surprising given that it is considered thermolabile, with an incubation temperature of 25°C often recommended

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Manufacturer protocol

Download the product protocol from Promega for SmaI R6121 below.

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