XbaI restriction enzyme

Restriction Enzymes XbaI

Experiment
Restriction Enzymes XbaI
Product
XbaI restriction enzyme from Takara Bio Inc
Manufacturer
Takara Bio Inc

Protocol tips

Protocol tips
All isolates were analyzed by using the restriction endonucleases BlnI (Takara) and XbaI (Takara).

Publication protocol

Pulsed-field gel electrophoresis (PFGE) typing of S. Enteritids was performed by the method of Izumiya et al. (8, 9). All isolates were analyzed by using the restriction endonucleases BlnI (Takara) and XbaI (Takara). PFGE was performed with a CHEF-DR II apparatus (Bio-Rad Laboratories, Richmond, CA) at 6 V/cm and 14°C. A linearly ramped switching time from 5 to 50 s was applied for 21 h. A Lambda phage ladder (Bio-Rad Laboratories) was used as a molecular size marker.

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Manufacturer protocol

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