EcoRI R6011

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	The clone 4G4E4.0 encompassing a 4-kb EcoRI 59 fragment of the human huntingtin gene (including exon 1 and upstream noncoding region) (Lin et al., 1995) was characterized by restriction endonuclease mapping with EcoRI, HindIII, and SfiI.

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The clone 4G4E4.0 encompassing a 4-kb EcoRI 59 fragment of the human huntingtin gene (including exon 1 and upstream noncoding region) (Lin et al., 1995) was characterized by restriction endonuclease mapping with EcoRI, HindIII, and SfiI.

Publication protocol

"The construction of an expression vector
containing the human huntingtin exon 1 with a normal range repeat
for in vitro transcription is summarized in Fig. 1. The clone 4G4E4.0
encompassing a 4-kb EcoRI 59 fragment of the human huntingtin
gene (including exon 1 and upstream noncoding region) (Lin et al.,
1995) was characterized by restriction endonuclease mapping with
EcoRI, HindIII, and SfiI. A region of 3345 nt of the 59-untranslated
region was deleted by double digestion with HindIII and SfiI to form
a human huntingtin exon 1 transcription plasmid (clone 839; Fig. 1).
An mRNA containing 222 nt of the 59-untranslated region of human
huntingtin exon 1 and an open reading frame that translates into a
10,068-Da huntingtin gene product corresponding to human exon 1
was used for in vitro expression driven by a T3 promoter (Fig. 1). The
Qiagen maxi-prep plasmid isolation system (Santa Clara, CA) was
used for plasmid preparation."

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