FastDigest EcoRI

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	The Fermentas FastDigestTM Restriction Enzyme EcoRI, which was specifically formulated to cleave DNA in just 5 min, was used in the experiment. The digestion media, which was prepared using 1 L of EcoRI (1 FDU/L) and 2 L enzyme reaction buffer (10 × FastDigestTM buffer), was placed on the dsDNA oligonucleotides immobilized microelectrode and incubated in a dark, humid environment for 5 min at 37 ◦C. Then the microelectrode surface was washed with Milli-Q water, dried under a stream of N2. Control probe DNA3 and complementary target DNA4 which were designed without specific recognition site of the EcoRI endonuclease were used under the same treatment. Enzyme reaction buffer was placed on the dsDNA immobilized microelectrode only as a control experiment.

Protocol tips

The Fermentas FastDigestTM Restriction Enzyme EcoRI, which was specifically formulated to cleave DNA in just 5 min, was used in the experiment. The digestion media, which was prepared using 1 L of EcoRI (1 FDU/L) and 2 L enzyme reaction buffer (10 × FastDigestTM buffer), was placed on the dsDNA oligonucleotides immobilized microelectrode and incubated in a dark, humid environment for 5 min at 37 ◦C. Then the microelectrode surface was washed with Milli-Q water, dried under a stream of N2. Control probe DNA3 and complementary target DNA4 which were designed without specific recognition site of the EcoRI endonuclease were used under the same treatment. Enzyme reaction buffer was placed on the dsDNA immobilized microelectrode only as a control experiment.

Publication protocol

The Fermentas FastDigestTM Restriction Enzyme EcoRI, which was specifically formulated to cleave DNA in just 5 min, was used in the experiment. The digestion media, which was prepared using 1 L of EcoRI (1 FDU/L) and 2 L enzyme reaction buffer (10 × FastDigestTM buffer), was placed on the dsDNA oligonucleotides immobilized microelectrode and incubated in a dark, humid environment for 5 min at 37 ◦C. Then the microelectrode surface was washed with Milli-Q water, dried under a stream of N2. Control probe DNA3 and complementary target DNA4 which were designed without specific recognition site of the EcoRI endonuclease were used under the same treatment. Enzyme reaction buffer was placed on the dsDNA immobilized microelectrode only as a control experiment

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