FastDigest HpaII

Protocol tips

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	DNA digestion reactions were set up with 200 ng DNA and 1 µL of each enzyme (AciI, MspI or HpaII FastDigest - purchased from Fermentas) in a total volume of 20 µL and incubated for 1 h at 37°C. Reaction products were loaded on 2% MetaPhor high resolution agarose (Lonza) gel with 0.4 µg/mL of Ethidium Bromide.

Protocol tips
DNA digestion reactions were set up with 200 ng DNA and 1 µL of each enzyme (AciI, MspI or HpaII FastDigest - purchased from Fermentas) in a total volume of 20 µL and incubated for 1 h at 37°C. Reaction products were loaded on 2% MetaPhor high resolution agarose (Lonza) gel with 0.4 µg/mL of Ethidium Bromide.

Publication protocol

"Testing controls (unmethylated and methylated DNA fragments) by methylation sensitive restriction enzyme assays.
DNA digestion reactions were set up with 200 ng DNA and
1 μL of each enzyme (AciI, MspI or HpaII FastDigest—
purchased from Fermentas) in a total volume of 20 μL and incubated for 1 h at 37°C. Reaction products were loaded on 2%MetaPhor high resolution agarose (Lonza) gel with 0.4 μg/mL
of Ethidium Bromide"

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Manufacturer protocol

Download the product protocol from Thermo Fisher Scientific for FastDigest HpaII below.

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