BglI (10 U/µL)

Protocol tips

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	PCR-RFLP method was used to detect Single Nucleotide Polymorphism (SNP) of ORF 38 and 54. The PstI and BglI restriction enzymes (Thermo Fisher Scientific Inc, Waltham, MA USA) was used for digestion reactions with the following protocol: 18 µl Nuclease-free water, 10 µl of PCR product, 2 µl of 10X endonuclease buffer 0, and 1 µl digestion enzymes PstI or BglI. Heating prepared at 37 ⁰C for 15 h. Visualization performed by 2% Agarose gel electrophoresis.

Protocol tips

PCR-RFLP method was used to detect Single Nucleotide Polymorphism (SNP) of ORF 38 and 54. The PstI and BglI restriction enzymes (Thermo Fisher Scientific Inc, Waltham, MA USA) was used for digestion reactions with the following protocol: 18 µl Nuclease-free water, 10 µl of PCR product, 2 µl of 10X endonuclease buffer 0, and 1 µl digestion enzymes PstI or BglI. Heating prepared at 37 ⁰C for 15 h. Visualization performed by 2% Agarose gel electrophoresis.

Publication protocol

PCR-RFLP method was used to detect Single Nucleotide Polymorphism (SNP) of ORF 38 and 54. The PstI and BglI restriction enzymes (Thermo Fisher Scientific Inc, Waltham, MA USA) was used for digestion reactions with the following protocol: 18 µl Nuclease-free water, 10 µl of PCR product, 2 µl of 10X endonuclease buffer 0, and 1 µl digestion enzymes PstI or BglI. Heating prepared at 37 ⁰C for 15 h. Visualization performed by 2% Agarose gel electrophoresis.

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