CellTiter 96® AQueous One Solution Cell Proliferation Assay (MTS)

Protocol tips

Upstream tips	Protocol tips	Downstream tips
- Thaw Reagent approximately for 90 minutes at room temperature, or 10 minutes in a water bath at 37°C.	- 20 μl of MTS is added to cells and incubated for 1 h.

Upstream tips
- Thaw Reagent approximately for 90 minutes at room temperature, or 10 minutes in a water bath at 37°C.
Protocol tips
- 20 μl of MTS is added to cells and incubated for 1 h.

Publication protocol

Cell viability was assessed using a CellTiter 96™ AQueous One Solution Cell Proliferation Assay (Promega, Madison, WI, USA). Briefly, SH-SY5Y cells were seeded into 96-well plates (2 × 104 cells/well) and cultivated overnight. The following day, cells were treated with the indicated concentrations of the appropriate drug(s) for varying lengths of time. After treatment, 20 μl of CellTiter 96 AQueous One Solution™ Reagent was added to each well, the plates were incubated at 37 °C for 1 h, and the absorbance of each sample was measured using an iMark™ Micropate Reader (BIO-RAD, Hercules, CA, USA) at a wavelength of 490 nm. Cell viability was then calculated by comparing the absorbance of treated cells with that of the control cells (incubated without drugs), which was defined as 100 % [13, 14]. All samples were tested in triplicate for each experiment.

Full paper   Login or join for free to view the full paper.

Reviews

Discussion

Papers

Check out relevant papers found by Labettor's AI that are relevant for performing Cell cytotoxicity / Proliferation assay cell type - SH-SY5Y using CellTiter 96® AQueous One Solution Cell Proliferation Assay (MTS) from Promega.

Manufacturer protocol

Download the product protocol from Promega for CellTiter 96® AQueous One Solution Cell Proliferation Assay (MTS) below.

Download manufacturer protocol

Videos

Check out videos that might be relevant for performing Cell cytotoxicity / Proliferation assay cell type - SH-SY5Y using CellTiter 96® AQueous One Solution Cell Proliferation Assay (MTS) from Promega. Please note that these videos are representative and steps or experiment specific processes must be kept in mind to expect desired results.