BlnI (AvrII) restriction enzyme

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	Pulsed-field gel electrophoresis (PFGE) typing of S. Enteritids was performed by the method of Izumiya et al. (8, 9). All isolates were analyzed by using the restriction endonucleases BlnI (Takara) and XbaI (Takara). PFGE was performed with a CHEF-DR II apparatus (Bio-Rad Laboratories, Richmond, CA) at 6 V/cm and 14°C. A linearly ramped switching time from 5 to 50 s was applied for 21 h. A Lambda phage ladder (Bio-Rad Laboratories) was used as a molecular size marker.

Protocol tips

Pulsed-field gel electrophoresis (PFGE) typing of S. Enteritids was performed by the method of Izumiya et al. (8, 9). All isolates were analyzed by using the restriction endonucleases BlnI (Takara) and XbaI (Takara). PFGE was performed with a CHEF-DR II apparatus (Bio-Rad Laboratories, Richmond, CA) at 6 V/cm and 14°C. A linearly ramped switching time from 5 to 50 s was applied for 21 h. A Lambda phage ladder (Bio-Rad Laboratories) was used as a molecular size marker.

Publication protocol

"Pulsed-field gel electrophoresis (PFGE) typing of S. Enteritids was performed by the method of Izumiya et al. (8, 9). All isolates were analyzed by using the restriction endonucleases BlnI (Takara) and XbaI (Takara). PFGE was performed with a CHEF-DR II apparatus (Bio-Rad Laboratories, Richmond, CA) at 6 V/cm and 14°C. A linearly ramped switching time from 5 to 50 s was applied for 21 h. A Lambda phage ladder (Bio-Rad Laboratories) was used as a molecular size marker.

A dendrogram was constructed from the PFGE profiles according to the Dice coefficient and an unweighted pair-group method with arithmetic averaging cluster analysis was performed with Finger-Printing II software (Bio-Rad Laboratories). Strains showing more than 80% similarity were defined as the same subtype."

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