FastDigest XmaJI

Protocol tips

Upstream tips	Protocol tips	Downstream tips
	The tobacco mosaic virus based pJL-TRBO vector29 was used in this study. The pJL-TRBO vector and the synthesized vectors, containing the Mb sequences, were digested with PacI and XmaJI FastDigest restriction enzymes (Thermo Fisher Scientific).

Protocol tips
The tobacco mosaic virus based pJL-TRBO vector29 was used in this study. The pJL-TRBO vector and the synthesized vectors, containing the Mb sequences, were digested with PacI and XmaJI FastDigest restriction enzymes (Thermo Fisher Scientific).

Publication protocol

The tobacco mosaic virus based pJL-TRBO vector29 was used in this study. The pJL-TRBO vector and the synthesized vectors, containing the Mb sequences, were digested with PacI and XmaJI FastDigest restriction enzymes (Thermo Fisher Scientific). The Mb fragments were isolated using an agarose gel and purified using a gel extraction kit (Thermo Fisher Scientific). The Mb sequences were then cloned into the pJL-TRBO vector and transformed into competent cells of E. coli (Takara Bio, Kusatsu, Japan) following the manufacturers’ protocol. The bacteria were then cultured on the Luria-Bertani (LB) medium with kanamycin for selection and the presence of the ligated vectors were confirmed by colony PCR using vector specific primers. The PCR positive plasmids were further confirmed by sequencing by Eurofins Genomics (Ebersberg, Germany) and then transformed into competent cells of A. tumefaciens GV3101:pMP90 by electroporation for further use.

Full paper   Login or join for free to view the full paper.

Reviews

Discussion

Papers

Check out relevant papers found by Labettor's AI that are relevant for performing Restriction Enzymes BlnI / AvrII / XmaJI using FastDigest XmaJI from Thermo Fisher Scientific.

Manufacturer protocol

Download the product protocol from Thermo Fisher Scientific for FastDigest XmaJI below.

Download manufacturer protocol

Videos

Check out videos that might be relevant for performing Restriction Enzymes BlnI / AvrII / XmaJI using FastDigest XmaJI from Thermo Fisher Scientific. Please note that these videos are representative and steps or experiment specific processes must be kept in mind to expect desired results.