MboII (5 U/µL)

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	Exon 1 (A/D, codon 52, rs5030737; A/B, codon 54, rs1800450 and A/C, codon 57, rs1800451) polymorphisms were investigated with the use of PCR–RFLP procedures, employing MluI, BshNI and MboII (all purchased from Thermo Fisher Scientific) enzymes, respectively66.

Protocol tips
Exon 1 (A/D, codon 52, rs5030737; A/B, codon 54, rs1800450 and A/C, codon 57, rs1800451) polymorphisms were investigated with the use of PCR–RFLP procedures, employing MluI, BshNI and MboII (all purchased from Thermo Fisher Scientific) enzymes, respectively66.

Publication protocol

Polymorphisms of the FCN2 gene: − 64 A > C (rs78654553), (located within promoter), + 6,359 C > T (rs175491193), and + 6,424 G > T (rs7851696) (both exon 8) were analysed using allele-specific PCR, as described previously64. Another promoter polymorphism, − 4 A > G (rs17514136) was investigated using PCR–RFLP procedure, employing MboII restriction enzyme (Thermo Fisher Scientific)64.

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