Cell Counting Kit-8

Protocol tips

Upstream tips	Protocol tips	Downstream tips
	- Forty-eight hours after transfection, add 10 μl of CCK-8 solution to each well of the plate

Protocol tips
- Forty-eight hours after transfection, add 10 μl of CCK-8 solution to each well of the plate

Publication protocol

HepG2 cells seeded at a density of 3×104 per well into 96-well plates were transfected with miR-222 mimic (50 nM), inhibitor (100 nM) or their negative controls. Forty-eight hours after transfection, a cell proliferation assay was performed using Cell Counting Kit-8 (CCK-8) (Dojindo, Japan) according to the manufacturer’s instructions. The absorbance was read at 450 nm with a microplate reader (Bio-Rad)

Full paper   Login or join for free to view the full paper.

Reviews

Discussion

Papers

Check out relevant papers found by Labettor's AI that are relevant for performing Cell cytotoxicity / Proliferation assay cell type - Hep G2 using Cell Counting Kit-8 from Dojindo.

Manufacturer protocol

Download the product protocol from Dojindo for Cell Counting Kit-8 below.

Download manufacturer protocol

Videos

Check out videos that might be relevant for performing Cell cytotoxicity / Proliferation assay cell type - Hep G2 using Cell Counting Kit-8 from Dojindo. Please note that these videos are representative and steps or experiment specific processes must be kept in mind to expect desired results.