HinfI restriction enzyme

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	According to the SNP mutation site gene, we selected the restriction enzyme HinfI (Takara Bio, Inc.) for the treatment of PCR amplified fragments, to carry on the identification of the gene. All of the 180 GDM and 210 in the control group PCR amplified fragments were by HinfI. The reaction system contained Takara HinfI 1 μl, 10X HinfI buffer 2 μl, PCR template 7-8 μl, add ultrapure water to 20 μl, kept in 37°C water for 8 h. The fragments digested by HinfI were electrophoresed in a 3% agarose gel. Genotypes were determined by the results of various bands though UV light (c150; Azure Biosystems, Inc.).

Protocol tips

According to the SNP mutation site gene, we selected the restriction enzyme HinfI (Takara Bio, Inc.) for the treatment of PCR amplified fragments, to carry on the identification of the gene. All of the 180 GDM and 210 in the control group PCR amplified fragments were by HinfI. The reaction system contained Takara HinfI 1 μl, 10X HinfI buffer 2 μl, PCR template 7-8 μl, add ultrapure water to 20 μl, kept in 37°C water for 8 h. The fragments digested by HinfI were electrophoresed in a 3% agarose gel. Genotypes were determined by the results of various bands though UV light (c150; Azure Biosystems, Inc.).

Publication protocol

"Restriction enzyme digestion technique. According to the SNP mutation site gene, we selected the restriction enzyme HinfI (Takara Bio, Inc.) for the treatment of PCR amplified fragments, to carry on the identification of the gene. All of the 180 GDM and 210 in the control group PCR amplified fragments were by HinfI. The reaction system contained Takara HinfI 1 μl, 10X HinfI buffer 2 μl, PCR template 7-8 μl, add ultrapure water to 20 μl, kept in 37̊C water for 8 h. The fragments digested by HinfI were electrophoresed in a 3% agarose
gel. Genotypes were determined by the results of various bands though UV light (c150; Azure Biosystems, Inc.)."

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