DdeI R6291

Protocol tips

Upstream tips	Protocol tips	Downstream tips
	Aliquots of 10 μL of each 275 bp amplified product were digested with restriction endonucleases as recommended by the manufacturer. Three restriction enzymes, DdeI, HaeIII and HinfI (Promega, Madison, WI, USA), were used in separate reactions.

Protocol tips
Aliquots of 10 μL of each 275 bp amplified product were digested with restriction endonucleases as recommended by the manufacturer. Three restriction enzymes, DdeI, HaeIII and HinfI (Promega, Madison, WI, USA), were used in separate reactions.

Publication protocol

Restriction digest of the amplified DNA - Aliquots of 10 μL of each 275 bp amplified product were digested with restriction endonucleases as recommended by the manufacturer. Three restriction enzymes, DdeI, HaeIII and HinfI (Promega, Madison, WI, USA), were used in separate reactions.

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Manufacturer protocol

Download the product protocol from Promega for DdeI R6291 below.

Download manufacturer protocol

Videos

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