CellTiter 96® AQueous One Solution Cell Proliferation Assay (MTS)

Protocol tips

Upstream tips	Protocol tips	Downstream tips
- Thaw One Solution Reagent before use.		- Absorbance is recorded at 492nm

Upstream tips
- Thaw One Solution Reagent before use.
Downstream tips
- Absorbance is recorded at 492nm

Publication protocol

PCa cells were plated into 96-well plates at a concentration of 4,000 cells / 100 μl / well in growth medium. In some cases (Figure 3A and B), cells were cultured in different serum conditions (1% FBS and 10% FBS). Proliferation was quantified using One Solution Cell Proliferation Assay kit (Promega, Cat #: G3580), which contains a tetrazolium compound [3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium, inner salt; MTS]. At different time points (24h and 48h), optical intensities were read on a multiwell scanning spectrophotometer at OD 492 nm (Thermo Labsystems). An automatic blanking measurement was performed immediately before a measurement, and Performance Verification kit (Thermo Labsystems, Cat #: 24071960) was used to check the photometric accuracy. Cells cultured in medium with 10%FBS served as positive control. Cells cultured in serum-free medium served as negative control.

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Manufacturer protocol

Download the product protocol from Promega for CellTiter 96® AQueous One Solution Cell Proliferation Assay (MTS) below.

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