AluI restriction enzyme

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	Five microliters of each sample was digested with restriction enzyme AluI (Takara Shuzo).

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Five microliters of each sample was digested with restriction enzyme AluI (Takara Shuzo).

Publication protocol

The following primers were used for the PCR: m6-DNA-PK(+), 5′-GGAAAAGAATTGGTATCCAC-3′ (Fig. 1, primer o); and m8-DNA-PK(−), 5′-GTTGGCCCCTGCTAACTTTC-3′ (Fig. 1, primer p). The template DNAs were prepared from the spleen of SCID and C.B-17 mice purchased from Nippon Clea (Tokyo) by using SepaGene Kit (Sanko-jyunyaku, Tokyo). The subjects were 4-week-old female mice. One microgram of cellular DNA fractions was used for template. The amplification reaction was carried out in a 50 μl volume in the presence of 200 μM dNTPs, 0.4 μM primer, 50 mM KCl, 1.5 mM MgCl2, 10 mM Tris·HCl (pH 8.3), and 1.25 units of Taq DNA polymerase (Takara Shuzo). Five microliters of each sample was digested with restriction enzyme AluI (Takara Shuzo).

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