Cell Counting Kit-8

Protocol tips

Upstream tips	Protocol tips	Downstream tips
- After 24 h, 10 μl of solution from CCK-8 was added to each well. - Plates were incubated for 45 minutes in a humidified CO2 incubator at 37°C

Upstream tips
- After 24 h, 10 μl of solution from CCK-8 was added to each well. - Plates were incubated for 45 minutes in a humidified CO2 incubator at 37°C

Publication protocol

To investigate the effects of GOLPH3 on the rate of cell proliferation, we used Cell Counting Kit-8 (CCK-8) (Dojindo Laboratories, Kumamoto, Japan). Cells (3×103 cells/well) were first seeded into 96-well flat-bottom plates for 8 h to adhere. After 24 h, 10 μl of solution from CCK-8 was added to each well. These plates were incubated for 45 minutes in a humidified CO2 incubator at 37°C. Finally, the absorbance of sample taken from each well was measured at 450 nm, on the basis of which the percentage of surviving cells in each treatment group was plotted relative to the untreated one.

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Manufacturer protocol

Download the product protocol from Dojindo for Cell Counting Kit-8 below.

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