FastDigest FokI

Protocol tips

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	After the production of PCR products, FokI and ApaI restriction enzymes (Thermo Fisher Scientific, USA) and enzyme buffer were used for digestion of PCR products (RFLP or Restriction fragment length polymorphism). FokI PCR product was digested for 2 hours in 55°C, ApaI PCR product was digested for 2 hours in 37°C, and electrophoresis of digestion products on 2.5% agarose gel was used to determine FokI and ApaI polymorphisms. For FokI site, 265 bp and 169+96 bp represented alleles C and T, respectively (Fig. 1). For ApaI, 740 bp and 530+210 bp represented alleles A and C, respectively (Fig. 2).

Protocol tips

After the production of PCR products, FokI and ApaI restriction enzymes (Thermo Fisher Scientific, USA) and enzyme buffer were used for digestion of PCR products (RFLP or Restriction fragment length polymorphism). FokI PCR product was digested for 2 hours in 55°C, ApaI PCR product was digested for 2 hours in 37°C, and electrophoresis of digestion products on 2.5% agarose gel was used to determine FokI and ApaI polymorphisms. For FokI site, 265 bp and 169+96 bp represented alleles C and T, respectively (Fig. 1). For ApaI, 740 bp and 530+210 bp represented alleles A and C, respectively (Fig. 2).

Publication protocol

After the production of PCR products, FokI and ApaI restriction enzymes (Thermo Fisher Scientific, USA) and enzyme buffer were used for digestion of PCR products (RFLP or Restriction fragment length polymorphism). FokI PCR product was digested for 2 hours in 55°C, ApaI PCR product was digested for 2 hours in 37°C, and electrophoresis of digestion products on 2.5% agarose gel was used to determine FokI and ApaI polymorphisms. For FokI site, 265 bp and 169+96 bp represented alleles C and T, respectively (Fig. 1). For ApaI, 740 bp and 530+210 bp represented alleles A and C, respectively (Fig. 2).

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