DraI (10 U/µL)

Protocol tips

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	Agarose plugs were cut with a sterile scalpel to fit the size of the combs of the gel casting (3-4 mm). They were washed in restriction buffer at 4°C for 30 minutes. The restriction enzymes (REs) used for PFGE sample preparation included DraI and XbaI (Fermentas, Canada) with the appropriate restriction buffer. The restriction digestion steps were done separately by adding 30 U of XbaI enzyme followed by incubation at 37°C for four hours and DraI enzyme followed by an overnight incubation at 37°C.

Protocol tips

Agarose plugs were cut with a sterile scalpel to fit the size of the combs of the gel casting (3-4 mm). They were washed in restriction buffer at 4°C for 30 minutes. The restriction enzymes (REs) used for PFGE sample preparation included DraI and XbaI (Fermentas, Canada) with the appropriate restriction buffer. The restriction digestion steps were done separately by adding 30 U of XbaI enzyme followed by incubation at 37°C for four hours and DraI enzyme followed by an overnight incubation at 37°C.

Publication protocol

Agarose plugs were cut with a sterile scalpel to fit the size of the combs of the gel casting (3-4 mm). They were washed in restriction buffer at 4°C for 30 minutes. The restriction enzymes (REs) used for PFGE sample preparation included DraI and XbaI (Fermentas, Canada) with the appropriate restriction buffer. The restriction digestion steps were done separately by adding 30 U of XbaI enzyme followed by incubation at 37°C for four hours and DraI enzyme followed by an overnight incubation at 37°C.

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