MnlI (10 U/µL)

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	The product was digested using MnlI (Thermo Fisher Scientific, MA, USA)

Protocol tips
The product was digested using MnlI (Thermo Fisher Scientific, MA, USA)

Publication protocol

"Analysis of SNP in the promoter region of the IL-10 at
sites (
1082,
819 and
592)
1.4.1.1. IL-10 (
1082 G/A) (rs1800896). Genotyping was
done using polymerase chain reaction–restriction fragment
length polymorphism (PCR–RFLP) assay as previously reported [16,17]. An amplification product 196 bp was obtained using standard PCR assay, according to cycling conditions consisted of an initial denaturation step at 94 C for 2 min, followed by 40 cycles of 94 C for 30 s, 59 C for 55 s and 72 C for 45 s, with a final extension of 72 C for 5 min. The product was digested using MnlI (Thermo Fisher Scientific, MA, USA)"

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