XmiI (AccI) (10 U/µL)

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	The PCR products were digested using XmiI (AccI; Thermo Scientific, Germany) at 37C for 3 h. The digested PCR products were separated by 2% agarose gel electrophoresis and stained with Power Load (Kawsar Biotech, Iran).

Protocol tips
The PCR products were digested using XmiI (AccI; Thermo Scientific, Germany) at 37C for 3 h. The digested PCR products were separated by 2% agarose gel electrophoresis and stained with Power Load (Kawsar Biotech, Iran).

Publication protocol

DNA was extracted from peripheral blood using a standard salting-out method. For genotyping of Val109Asp (rs2274907), a polymerase chain reaction (PCR) DNA-restriction fragment length polymorphism technique was used. We used forward 50 -GAG CCTTTAGGCCATGTCTCT-30 and reverse R 50 -TCTCTCTTCTTCTCCAGCCCAT-30 primers to amplify the target sequence using a PCR. The reactions were performed with 20 mL of reaction mixture containing 0.4 mM of each primer, 200 mM of deoxynucleotide precursors, 2 mM MgCl2, and 1 unit of Taq DNA polymerase (Amplicon, Denmark). The PCR products were digested using XmiI (AccI; Thermo Scientific, Germany) at 37C for 3 h. The digested PCR products were separated by 2% agarose gel electrophoresis and stained with Power Load (Kawsar Biotech, Iran). The PCR program and details of digestion have been described elsewhere

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