AccI restriction enzyme

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	Plasmid DNA from transformants was subjected to restriction endonuclease digestion using the enzymes AccI (Takara, Tokyo, Japan), HincII (Takara), PvuI (Thermo Scientific) and XhoI (Thermo Scientific) followed by agarose gel electrophoresis.

Protocol tips
Plasmid DNA from transformants was subjected to restriction endonuclease digestion using the enzymes AccI (Takara, Tokyo, Japan), HincII (Takara), PvuI (Thermo Scientific) and XhoI (Thermo Scientific) followed by agarose gel electrophoresis.

Publication protocol

Plasmid DNA from transformants was subjected to restriction endonuclease digestion using the enzymes AccI (Takara, Tokyo, Japan), HincII (Takara), PvuI (Thermo Scientific) and XhoI (Thermo Scientific) followed by agarose gel electrophoresis. The ethidium bromide stained gel was visualized using a UV transilluminator.

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Manufacturer protocol

Download the product protocol from Takara Bio Inc for AccI restriction enzyme below.

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