Bsp68I (NruI) (10 U/µL)

Protocol tips

Upstream tips	Protocol tips	Downstream tips
	Restriction digestion of the 300-bp product, obtained from PCR of all the clinical isolates, the reference strains, and the spiked sputum sample, was carried out with 1 U each of NruI and BamHI (Fermentas Life Sciences, Lithuania) in separate tubes. Restriction digestion was carried out with 10 μl of the amplicon at 37°C for 2 h.	The products were electrophoresed on 2.5% agarose gel with a 50-bp DNA marker (Fermentas Life Sciences, Lithuania).

Protocol tips
Restriction digestion of the 300-bp product, obtained from PCR of all the clinical isolates, the reference strains, and the spiked sputum sample, was carried out with 1 U each of NruI and BamHI (Fermentas Life Sciences, Lithuania) in separate tubes. Restriction digestion was carried out with 10 μl of the amplicon at 37°C for 2 h.
Downstream tips
The products were electrophoresed on 2.5% agarose gel with a 50-bp DNA marker (Fermentas Life Sciences, Lithuania).

Publication protocol

Restriction digestion of the 300-bp product, obtained from PCR of all the clinical isolates, the reference strains, and the spiked sputum sample, was carried out with 1 U each of NruI and BamHI (Fermentas Life Sciences, Lithuania) in separate tubes. Restriction digestion was carried out with 10 μl of the amplicon at 37°C for 2 h. The products were electrophoresed on 2.5% agarose gel with a 50-bp DNA marker (Fermentas Life Sciences, Lithuania).

Full paper   Login or join for free to view the full paper.

Reviews

Discussion

Papers

Check out relevant papers found by Labettor's AI that are relevant for performing Restriction Enzymes NruI / Bsp68I using Bsp68I (NruI) (10 U/µL) from Thermo Fisher Scientific.

Manufacturer protocol

Download the product protocol from Thermo Fisher Scientific for Bsp68I (NruI) (10 U/µL) below.

Download manufacturer protocol

Videos

Check out videos that might be relevant for performing Restriction Enzymes NruI / Bsp68I using Bsp68I (NruI) (10 U/µL) from Thermo Fisher Scientific. Please note that these videos are representative and steps or experiment specific processes must be kept in mind to expect desired results.