BbvI NEB#R0173

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	Escherichia coli genomic DNA was sourced from the MG1655 strain supplied from the American Type Culture Collection (Manassas, VA). DNA was isolated by lysis of bacterial cells in an SDS–proteinase K solution followed by RNAse treatment and multiple phenol/chloroform extractions before ethanol precipitation (16). Human genomic DNA was purchased from Promega (Madison, WI). The DNA (10 µg) was digested with 8 U of the restriction enzyme BbvI (NEB) for 3 h at 37°C before heat inactivation at 65°C for 20 min.

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Escherichia coli genomic DNA was sourced from the MG1655 strain supplied from the American Type Culture Collection (Manassas, VA). DNA was isolated by lysis of bacterial cells in an SDS–proteinase K solution followed by RNAse treatment and multiple phenol/chloroform extractions before ethanol precipitation (16). Human genomic DNA was purchased from Promega (Madison, WI). The DNA (10 µg) was digested with 8 U of the restriction enzyme BbvI (NEB) for 3 h at 37°C before heat inactivation at 65°C for 20 min.

Publication protocol

Escherichia coli genomic DNA was sourced from the MG1655 strain supplied from the American Type Culture Collection (Manassas, VA). DNA was isolated by lysis of bacterial cells in an SDS–proteinase K solution followed by RNAse treatment and multiple phenol/chloroform extractions before ethanol precipitation (16). Human genomic DNA was purchased from Promega (Madison, WI). The DNA (10 µg) was digested with 8 U of the restriction enzyme BbvI (NEB) for 3 h at 37°C before heat inactivation at 65°C for 20 min.

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