BrdU Cell Proliferation Assay

Protocol tips

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	- Serum-free medium containing BrdU is added into cells and observed for the next 16h

Protocol tips
- Serum-free medium containing BrdU is added into cells and observed for the next 16h

Publication protocol

SKOV-3 cells (750 cells/well) were seeded on a 96-well culture plate. The next day, PITX2A, -B, and -C constructs were transfected alone or in combination with β-catenin siRNA or in DKK1 pretreated cells. After 6 h, medium was discarded, washed with sterile PBS, and incubated in serum-free medium containing BrdU to assess its incorporation for the next 16 h (Calbiochem).The growth rate of cells was assessed by the BrdU incorporation assay kit (Calbiochem). Photometric detection was done with dual wavelength ELISA reader (Bio-Rad) at 450 nm wavelength. The background was subtracted when the resulting data were processed.

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