FastDigest ApaI

Protocol tips

Upstream tips	Protocol tips	Downstream tips
	2200 bp bands amplified from gDNA of five +B and five 0B mice were removed from the agarose gels, purified with QIAquick Gel Extraction Kit (Qiagen) and then digested with twelve restriction endonucleases (RE): ApaI, HindIII, Bsp120I, MfeI, HpaI, MspI, MboI, AluI, MseI, Ecl136II, HaeIII and SacI (FastDigest, Thermo Scientific, Waltham, MA USA) according to the manufacturer’s instructions. Digested DNA fragments were separated by agarose gel electrophoresis, visualized and photo-documented as above.

Protocol tips

2200 bp bands amplified from gDNA of five +B and five 0B mice were removed from the agarose gels, purified with QIAquick Gel Extraction Kit (Qiagen) and then digested with twelve restriction endonucleases (RE): ApaI, HindIII, Bsp120I, MfeI, HpaI, MspI, MboI, AluI, MseI, Ecl136II, HaeIII and SacI (FastDigest, Thermo Scientific, Waltham, MA USA) according to the manufacturer’s instructions. Digested DNA fragments were separated by agarose gel electrophoresis, visualized and photo-documented as above.

Publication protocol

"Restriction Fragment Length Polymorphism (RFLP) Analysis
2200 bp bands amplified from gDNA of five +B and five 0B mice were removed from the agarose gels, purified with QIAquick Gel Extraction Kit (Qiagen) and then digested with twelve restriction endonucleases (RE): ApaI, HindIII, Bsp120I, MfeI, HpaI, MspI, MboI, AluI, MseI, Ecl136II, HaeIII and SacI (FastDigest, Thermo Scientific, Waltham, MA USA) according to the manufacturer’s instructions. Digested DNA fragments were separated by agarose gel electrophoresis, visualized and photo-documented as above."

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Manufacturer protocol

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