MluI NEB#R0198

Protocol tips

Upstream tips	Protocol tips	Downstream tips
	Restriction enzymes MluI (R0198), SalI-HF (R3138) and XhoI (R0146) were from NEB. Buffer 3.1 (B7203; final concentration: 100 mM NaCl, 50 mM Tris-HCl, 10 mM MgCl2, 100 μg/ml BSA, pH 7.9) was used for MluI-, SalI- and MluI-, XhoI-double-digests. 1 μg of plasmid DNA or PCR product was incubated with 5 U of each restriction enzyme in a final volume of 30 μl for 1 h at 37°C. DNA fragments were separated on 0.8% Agarose (Sigma) gels and purified using the QIAquick gel purification Kit (Qiagen).

Protocol tips

Restriction enzymes MluI (R0198), SalI-HF (R3138) and XhoI (R0146) were from NEB. Buffer 3.1 (B7203; final concentration: 100 mM NaCl, 50 mM Tris-HCl, 10 mM MgCl2, 100 μg/ml BSA, pH 7.9) was used for MluI-, SalI- and MluI-, XhoI-double-digests. 1 μg of plasmid DNA or PCR product was incubated with 5 U of each restriction enzyme in a final volume of 30 μl for 1 h at 37°C. DNA fragments were separated on 0.8% Agarose (Sigma) gels and purified using the QIAquick gel purification Kit (Qiagen).

Publication protocol

Restriction enzymes MluI (R0198), SalI-HF (R3138) and XhoI (R0146) were from NEB. Buffer 3.1 (B7203; final concentration: 100 mM NaCl, 50 mM Tris-HCl, 10 mM MgCl2, 100 μg/ml BSA, pH 7.9) was used for MluI-, SalI- and MluI-, XhoI-double-digests. 1 μg of plasmid DNA or PCR product was incubated with 5 U of each restriction enzyme in a final volume of 30 μl for 1 h at 37°C. DNA fragments were separated on 0.8% Agarose (Sigma) gels and purified using the QIAquick gel purification Kit (Qiagen).

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