Protocol tips
Upstream tips |
Protocol tips |
Downstream tips |
|
The precipitated amplification products were digested with the tagging enzyme BsmFI in a 50-μl reaction mixture containing 0.2 mM each dNTP, 1× NEB Buffer 4, 1× bovine serum albumin (BSA), and 2 U of BsmFI (NEB) for 1 h at 65°C. Restriction products were filled in by the addition of 3 U of T4 polymerase (NEB) to the reaction mixture and incubated for 20 min at 12°C. The reaction mixture was diluted to a volume of 200 μl with LoTE, and the products were extracted with PC8 and precipitated as described above. |
|
Protocol tips |
The precipitated amplification products were digested with the tagging enzyme BsmFI in a 50-μl reaction mixture containing 0.2 mM each dNTP, 1× NEB Buffer 4, 1× bovine serum albumin (BSA), and 2 U of BsmFI (NEB) for 1 h at 65°C. Restriction products were filled in by the addition of 3 U of T4 polymerase (NEB) to the reaction mixture and incubated for 20 min at 12°C. The reaction mixture was diluted to a volume of 200 μl with LoTE, and the products were extracted with PC8 and precipitated as described above. |
Publication protocol
The precipitated amplification products were digested with the tagging enzyme BsmFI in a 50-μl reaction mixture containing 0.2 mM each dNTP, 1× NEB Buffer 4, 1× bovine serum albumin (BSA), and 2 U of BsmFI (NEB) for 1 h at 65°C. Restriction products were filled in by the addition of 3 U of T4 polymerase (NEB) to the reaction mixture and incubated for 20 min at 12°C. The reaction mixture was diluted to a volume of 200 μl with LoTE, and the products were extracted with PC8 and precipitated as described above.
Full paper
Login or
join for free to view the full paper.
Reviews
BsmFI NEB#R0572 from New England BioLabs has not yet been reviewed for this experiment
We'd love it if you would be the first to write a review!
Discussion
Start your discussion
Share your thoughts or question with experts in your field
Start a discussion
Papers
Check out relevant papers found by Labettor's AI that are relevant for performing Restriction Enzymes BsmFI / FaqI using BsmFI NEB#R0572 from New England BioLabs.
Manufacturer protocol
Download the product protocol from New England BioLabs for BsmFI NEB#R0572 below.
We haven't found the manufacturer protocol for this product yet.
Videos
Check out videos that might be relevant for performing Restriction Enzymes BsmFI / FaqI using BsmFI NEB#R0572 from New England BioLabs. Please note that these videos are representative and steps or experiment specific processes must be kept in mind to expect desired results.
We haven't found any additional videos for this experiment / product combination yet.