FastDigest DpnI

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	Point mutations were generated by site-directed mutagenesis with FastDigest DpnI (Fermentas, FD1704) using mutagenic primers for PCR, and the plasmids encoding the wild-type protein were used as the template. All constructs were confirmed by DNA sequencing.

Protocol tips
Point mutations were generated by site-directed mutagenesis with FastDigest DpnI (Fermentas, FD1704) using mutagenic primers for PCR, and the plasmids encoding the wild-type protein were used as the template. All constructs were confirmed by DNA sequencing.

Publication protocol

"The encoding sequences of RIG-I, MAVS, IKKε, TBK1, IRF3, ncOGT were amplified from the human- and mouse-derived cDNA
libraries and subsequently cloned into pCMV6 vectors. Wild-type MAVS and indicated mutants were cloned into T2A-mutated
pCDH-MCS-T2A-copGFP-MSCV vectors for lentivirus package. Point mutations were generated by site-directed mutagenesis
with FastDigest DpnI (Fermentas, FD1704) using mutagenic primers for PCR, and the plasmids encoding the wild-type protein
were used as the template. All constructs were confirmed by DNA sequencing."

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