Protocol tips
Upstream tips |
Protocol tips |
Downstream tips |
|
5 μl of the PCR product and 2 μl of the restriction enzyme were prepared by mixing 2 μl of 10x buffer, and sterile free water was added for a total volume of 20 μl. The tubes were incubated at 37°C for approximately 4 h. The digested PCR products were then separated on 2.5% agarose gel. |
|
Protocol tips |
5 μl of the PCR product and 2 μl of the restriction enzyme were prepared by mixing 2 μl of 10x buffer, and sterile free water was added for a total volume of 20 μl. The tubes were incubated at 37°C for approximately 4 h. The digested PCR products were then separated on 2.5% agarose gel. |
Publication protocol
To further discriminate between targeted animals, expected nucleotide sequences were restriction mapped with the Mapdraw program of DNASTAR (NY, USA). After testing, one restriction enzyme SspI (TakaRa) was selected to digest the PCR products in separate reactions. Briefly, 5 μl of the PCR product and 2 μl of the restriction enzyme were prepared by mixing 2 μl of 10x buffer, and sterile free water was added for a total volume of 20 μl. The tubes were incubated at 37°C for approximately 4 h. The digested PCR products were then separated on 2.5% agarose gel.
Full paper
Login or
join for free to view the full paper.
Reviews
SspI restriction enzyme from Takara Bio Inc has not yet been reviewed for this experiment
We'd love it if you would be the first to write a review!
Discussion
Start your discussion
Share your thoughts or question with experts in your field
Start a discussion
Papers
Check out relevant papers found by Labettor's AI that are relevant for performing Restriction Enzymes SspI using SspI restriction enzyme from Takara Bio Inc.
Videos
Check out videos that might be relevant for performing Restriction Enzymes SspI using SspI restriction enzyme from Takara Bio Inc. Please note that these videos are representative and steps or experiment specific processes must be kept in mind to expect desired results.
We haven't found any additional videos for this experiment / product combination yet.