CellTiter 96® AQueous One Solution Cell Proliferation Assay (MTS)

Protocol tips

Upstream tips	Protocol tips	Downstream tips
- Completely thaw the 20ml size One Solution Reagent approximately 90 minutes at room temperature, or 10 minutes in a water bath at 37°C.	- Cells were incubated with 20 μl of MTS for 3 h	- To measure the absorbance later, add 25µl of 10% SDS to each well to stop the reaction. Store SDS-treated plates protected from light in a humidified chamber at room temperature for up to 18 hours.

Upstream tips
- Completely thaw the 20ml size One Solution Reagent approximately 90 minutes at room temperature, or 10 minutes in a water bath at 37°C.
Protocol tips
- Cells were incubated with 20 μl of MTS for 3 h
Downstream tips
- To measure the absorbance later, add 25µl of 10% SDS to each well to stop the reaction. Store SDS-treated plates protected from light in a humidified chamber at room temperature for up to 18 hours.

Publication protocol

PC9, PC9-G, PC9-3-2, PC9-3-14, and A549 human lung cancer cell lines were seeded at 3 × 103 cells/well in 96-well plates and treated with gefitinib or AZD9291; 96 h later, cell proliferation was assessed using the CellTiter 96 AQueous One Solution Cell Proliferation Assay kit (Promega, Madison, WI, USA) according to the manufacturer's instructions. Three independent experiments were performed and used to determine mean half-maximal inhibitory concentration (IC50).

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Manufacturer protocol

Download the product protocol from Promega for CellTiter 96® AQueous One Solution Cell Proliferation Assay (MTS) below.

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