BglII restriction enzyme

Protocol tips

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	For cloning of the amplicon, both pDisplay and GS-BAP DNA fragments were digested by BglII and SalI (Takara, Japan) and the corresponding bands were gel purified by GeneJET™ Gel Extraction Kit (Fermentas, Lithuania).

Protocol tips
For cloning of the amplicon, both pDisplay and GS-BAP DNA fragments were digested by BglII and SalI (Takara, Japan) and the corresponding bands were gel purified by GeneJET™ Gel Extraction Kit (Fermentas, Lithuania).

Publication protocol

For cloning of the amplicon, both pDisplay and GS-BAP DNA fragments were digested by BglII and SalI (Takara, Japan) and the corresponding bands were gel purified by GeneJET™ Gel Extraction Kit (Fermentas, Lithuania). Subsequently, the purified insert fragment (GS-BAP) was cloned into the BglII-SalI restriction sites of pDisplay, downstream of HA-tag and in frame with myc epitope by T4 DNA ligase to make the final pDis-GS-BAP plasmid (Fig. 1C). All the cloning steps were performed according to the standard protocols in Escherichia coli DH5α (25). Primary confirmation of the recombinant plasmid was performed by restriction digestion analysis using FastDigest SalI and FastDigest HindIII (Thermo scientific, Lithuania) and the selected clone was sequenced at the sequencing facility of Pasteur Institute of Iran using T7-promoter and BGH-Reverse universal primers.

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Manufacturer protocol

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