Cell Proliferation Kit I (MTT)

Protocol tips

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- Seeded cells in a 6 well plate were treated with medium containing 10% FBS for 2 weeks

Upstream tips
- Seeded cells in a 6 well plate were treated with medium containing 10% FBS for 2 weeks

Publication protocol

The Cell Proliferation Reagent Kit I (MTT; Roche Applied Science) was used to test the cell viability. A549 and PC-9 cells plated in 96-well plates (3,000 cells/well) were transfected with si-PVT1. We estimated the cell viability every 24 hours according to the manufacturer's protocol. The MTT experiments were conducted in quadruplicate. For colony formation assay, transfected cells (n = 1200) were placed in 6-well plates and maintained in proper medium containing 10% FBS for 2 weeks. The medium was replaced every 4 days, and visible colonies were counted after 2 weeks.

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Manufacturer protocol

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